cover image: Ontogenetically distinct neutrophils differ in function and transcriptional profile in zebrafish

Ontogenetically distinct neutrophils differ in function and transcriptional profile in zebrafish

1 Dec 2023

Abstract The current view of hematopoiesis considers leukocytes on a continuum with distinct developmental origins, and which exert non-overlapping functions. However, there is less known about the function and phenotype of ontogenetically distinct neutrophil populations. In this work, using a photoconvertible transgenic zebrafish line; Tg( mpx :Dendra2), we selectively label rostral blood island-derived and caudal hematopoietic tissue-derived neutrophils in vivo during steady state or upon injury. By comparing the migratory properties and single-cell expression profiles of both neutrophil populations at steady state we show that rostral neutrophils show higher csf3b expression and migration capacity than caudal neutrophils. Upon injury, both populations share a core transcriptional profile as well as subset-specific transcriptional signatures. Accordingly, both rostral and caudal neutrophils are recruited to the wound independently of their distance to the injury. While rostral neutrophils respond uniformly, caudal neutrophils respond heterogeneously. Collectively, our results reveal that co-existing neutrophils populations with ontogenically distinct origin display functional differences.

Authors

Juan García-López, Alexandre Grimaldi, Zelin Chen, Claudio Meneses, Karina Bravo-Tello, Erica Bresciani, Alvaro Banderas, Shawn Burgess, Pedro P Hernández, Carmen Feijoo

Related Organizations

Pontificia Universidad Católica de Chile
Bibliographic Reference
Juan García-López, Alexandre Grimaldi, Zelin Chen, Claudio Meneses, Karina Bravo-Tello, et al.. Ontogenetically distinct neutrophils differ in function and transcriptional profile in zebrafish. Nature Communications, 2023, 14 (1), pp.4942. ⟨10.1038/s41467-023-40662-7⟩. ⟨hal-04256141⟩
DOI
https://doi.org/10.1038/s41467-023-40662-7
HAL Collection
['CNRS - Centre national de la recherche scientifique', 'UNICANCER', 'Institut Curie', 'Génétique et Biologie du Développement', 'Sorbonne Université', 'Université Paris sciences et lettres', 'Sorbonne Université 01/01/2018', 'Faculté de Santé de Sorbonne Université', 'Faculté des Sciences de Sorbonne Université', 'Médecine - Sorbonne Université', 'Institut Curie - PSL', 'Sorbonne Université - Texte Intégral', 'Alliance Sorbonne Université', 'Fondation pour la Recherche Médicale']
HAL Identifier
4256141
Institution
['Institut National de la Santé et de la Recherche Médicale', 'Institut Curie [Paris]']
Laboratory
Génétique et Biologie du Développement
Published in
France

Table of Contents