Hsp70 progress report March 2016

Hsp70 progress report March 2016                  Department  of  Neurobiology  and  Anatomy                     Blazeman  Foundation  for  ALS   Progress  Report:    Administration  of  Hsp70  maintains  muscle  innervation  in  the  SOD1  mouse- ‐  a  new   therapeutic  approach?   Principal  Investigator:    Carol  Milligan,  Ph. [...]                           In  the  photo  on  left  (from  left  to  right):    Jane  Strupe,  Carol  Milligan,  Rachel  Gillespie,  Phonepasong  (Pop)  Arounleut,  Leea   Richardson,  Miles  Lyon,  Marlena  Wosiski- ‐  Kuhn. [...]       RhHsc70  Injections  in  Experimental  Animals   Summary:    We  are  performing  dose  response  experiments  to  determine  optimal  dose  of  recombinant  protein   to  delay  NMJ  denervation. [...]       We  have  injected  a  small  cohort  of  8  animals  with  previously  purified  rhHsc70,  dissected  out  the  muscles  of   interest,  and  are  in  the  process  of  examining  the  effects  of  the  protein  on  the  denervation  of  the   neuromuscular  junction. [...]     Substrate  Binding  Domain  Purification   Following  the  optimization  of  the  SOP  for  the  production  of  full- ‐length  protein,  we  will  begin  adapting  the   purification  for  the  substrate- ‐binding  domain  (SBD)  and  the  ATPase  domain  of  the  protein.