While highly effective at generating protein at high purity, the buffer system we used previously utilized a series of harsh chemicals that lead to rapid protein aggregation during renaturing when attempting to upscale to larger batches of protein. [...] Following confirmation of active protein, we next assessed rhHsc70’s therapeutic viability in preserving neuromuscular junction innervation in the SOD1G93A mouse model of ALS. [...] Previously, we have shown that systemic administration of recombinant human Hsp70 beginning at postnatal day 30 delays peripheral muscle denervation in this mouse model (Gifondorwa et al., 2012). [...] Future Directions We are performing a final series of experiments where the protein is being administered by intramuscular injection. [...] On behalf of all members of our ALS Center, we continue to express our gratitude to the Blazeman Foundation - ‐ because of this support we continue as Jon asked, we choose to "Believe...
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