THE DIVERSITY OF HALOTOLERANT AND HALOPHILIC BACTERIA IN THE SOIL OF THE NASINUAN SECONDARY FOREST IN MAHA SARAKHAM, THAILAND

Journal of Sustainability Science and Management eISSN: 2672-7226 Volume 16 Number 2, February 2021: 165-175 © Penerbit UMT THE DIVERSITY OF HALOTOLERANT AND HALOPHILIC BACTERIA IN THE SOIL OF THE NASINUAN SECONDARY FOREST IN MAHA SARAKHAM, THAILAND CHOOKIETWATTANA KANNIKA* AND YUWA-AMORNPITAK THALISA The Department of Biotechnology, Faculty of Technology, Mahasarakham University, Thailand. [...] It was facilitate their exploitation for commercial and thus aimed in this work to explore the diversity agricultural purposes (Kushner, 1993; Azua- of halotolerant and halophilic bacteria in the Chookietwattana Kannika and Yuwa-Amornpitak Thalisa 166 Nasinuan Secondary Forest and to obtain Phenotypic and Genotypic Characterization of halotolerant and halophilic bacterial resources Bacterial Isola. [...] For for the isolation of halotolerant bacteria, slightly testing growth at various temperatures, the halophilic bacteria, and moderately halophilic medium was kept at a pH level of 7.2 and NaCl bacteria according to the classification of at the optimum concentration of each isolate microorganisms in response to salt (NaCl) in was used. [...] The teichoic acids found in the cell walls of Gram-positive bacteria provide the negative Results and Discussion charges of the cell surface as a whole, thus Isolation of Bacteria from Soil Samples and increasing the mobility and transport of these Phenotypic Characterization bacteria in the environment. [...] As such, the halobacteria media with the final NaCl From the preliminary phenotypic concentration at 0%, 3%, and 6% w/v NaCl observation (data not shown), 16 out of the 55 Journal of Sustainability Science and Management Volume 16 Number 2, February 2021: 165-175 Chookietwattana Kannika and Yuwa-Amornpitak Thalisa 168 Figure 1: The results of microscopic observation of the Gram test isolates were.