International Journal o f - Molecular Sciences Tree Genetic Engineering, Genome

blackberry/raspberry, and the topics comprise the use of the CRISPR/Cas12a system in Tree Genetic Engineering, Genome different tree species, e.g., to knockout lignin and cellulose synthesis biosynthesis genes Editing and Genomics. [...] Yinxuan Xue and colleagues show in their paper “Investigation of PtSGT1 and PtSGT4 Function in Cellulose Biosynthesis in Populus tomentosa Using CRISPR/Cas9 Technology” that sucrose and fructose were significantly downregulated in the stems and leaves of mutant plants; however, the glucose levels did not change significantly in roots and stems of PtSGT1 mutants. [...] presents in “Genome-Wide Identification, Structure Characterization, and Expression Pattern Profiling of the Aquaporin Gene Family in Betula pendula” the first detailed genome-wide analysis of the AQP gene family in a birch species. [...] The authors contribute to a better understanding of the specific functions of the BpeAQP genes in the responses of silver birch trees to cold stress. [...] The authors selected representative cultivars of blackberry and raspberry for RT-qPCR combined with different internal stability analysis software programs and identified the two most stable genes (RuEEF1A and Ru18S), which can be used as reference genes for future studies of the analysis of differential gene expression in blackberry and raspberry.