Authors
Nadège Bossuet, Cécile Guyonnet, Camille Chagneau, Min Tang-Fichaux, Marie Penary, Dorian Loubet, Priscilla Branchu, Eric Oswald, Jean-Philippe Nougayrede
- Bibliographic Reference
- Nadège Bossuet, Cécile Guyonnet, Camille Chagneau, Min Tang-Fichaux, Marie Penary, et al.. Oxygen concentration modulates colibactin production. Gut microbes, 2023, 15 (1), pp.2222437. ⟨10.1080/19490976.2023.2222437⟩. ⟨hal-04147154⟩
- DOI
- https://doi.org/10.1080/19490976.2023.2222437
- HAL Collection
- ['INSERM - Institut national de la santé et de la recherche médicale', 'Université Paul Sabatier - Toulouse III', 'Archive ouverte en agrobiosciences', 'Institut National de Recherche en Agriculture, Alimentation et Environnement', 'Institut de recherche en santé digestive', 'INRAE Occitanie Toulouse', 'MICA-Unités', 'Toulouse - INP', 'Université Toulouse 3', 'Université Toulouse III / Toulouse INP', 'Ecole Nationale Vétérinaire de Toulouse']
- HAL Identifier
- 4147158
- Institution
- ['Université Toulouse III - Paul Sabatier', 'Institut National Polytechnique (Toulouse)', 'Institut National de la Santé et de la Recherche Médicale', 'Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement', 'Centre Hospitalier Universitaire de Toulouse']
- Laboratory
- Institut de Recherche en Santé Digestive
- Published in
- France
Table of Contents
- Abstract 3
- Introduction 3
- Results 4
- <italic>Oxygen inhibits colibactin autotoxicity in a colibactin-producing</italic> E. coli clbS <italic>mutant</italic> 4
- <italic>Oxygen inhibits DNA-crosslinking by</italic> pks<italic>+</italic> E. coli 4
- <italic>Oxygen inhibits the expression and production of colibactin by</italic> pks+ E. coli 5
- <italic>ArcA is required for full DNA-crosslinking by</italic> E. coli <italic>producing colibactin</italic> 7
- <italic>ArcA positively regulates the expression of</italic> clbB 7
- <italic>Oxygen and</italic> arcA <italic>mutation inhibit the genotoxicity of</italic> pks+ E. coli <italic>infecting epithelial cells</italic> 9
- Discussion 11
- Materials and methods 13
- Bacterial strains, mutants, plasmids and primers 13
- Bacterial culture conditions 14
- <italic>Exogenous DNA cross-linking by colibactin-producing</italic> E. coli 14
- Quantification of N-myristoyl-D-AsnOH (C14-Asn) 14
- <italic>Bioluminescence monitoring of</italic> clbB <italic>and</italic> clbS <italic>promoter activity</italic> 14
- clbB <italic>mRNA quantification</italic> 14
- Epithelial cells infection, quantification of DNA-damage and megalocytosis assay 15
- Purification, phosphorylation of ArcA−6His protein and electrophoretic mobility shift assay (EMSA) 15
- Statistical analyses 16
- Acknowledgments 16
- Disclosure statement 16
- Funding 16
- ORCID 16
- Data availability statement 16
- References 16
