STRENDA_list_level1A_1_7_

STRENDA Guidelines Level 1A Version 1.7 doi:10.3762/strenda.17 as approved on 22nd September 2016 The STRENDA Commission (Standards for Reporting Enzymology Data) compiled the following Guidelines, as a service to the community, to define the minimum amount of information that should accompany any published enzyme activity data. [...] The current STRENDA Guidelines (List Level 1A) was reviewed on the STRENDA meeting in September 2016 in terms of consistency of form and content, as well as of the order and plausibility of the list entries. [...] Data Comments Identity of the enzyme Name of reaction catalyst name, preferably the accepted name from the IUBMB Enzyme List EC number Sequence accession number Organism/species & strain NCBI Taxonomy ID Additional information on the enzyme Isoenzyme naturally occurring variant Tissue Organelle Localization within cell. [...] Methodology Assay method a literature reference may suffice for an established procedure but any modification should be detailed Type of assay e.g., continuous or discontinuous, direct or coupled Reaction stopping procedure in the case of discontinuous assays Direction of the assay with respect to the reaction equation provided e.g., NAD reduction by alcohol dehydrogenase; alcohol + NAD+ -> aldehy. [...] 1.7, September 2016 3 15/12/16 STRENDA Guidelines Level 1A Data Comments Reactant determined e.g., NADH formation, O2 utilization Additional material desirable Free metal cation concentrations e.g., of Mg2+ and Ca2+, specify how calculated Reaction equilibrium constant define conditions and reaction direction About the STRENDA Commission: The Commission was founded in 2003 and is supported by the.