Altered germline cyst formation and oogenesis in Tex14 mutant mice

When we examined the pattern of detected by antibody staining, while RacGAP-positive bridges were RacGAP and TEX14 distribution from the lateral face of the bridges observed at a decreased frequency (19.8±5.1 germ cells/bridge at in E12.5 wild-type ovaries, RacGAP distributed on the outer rim E12.5; 18.3±12.8 germ cells/bridge at E14.5) compared with the of the bridge that associate with the cell. [...] When observing the bridge from the lateral alter the expression of other cytokinesis proteins (MKLP1, ALIX, face, RacGAP was exclusively enriched in the inner layer of CEP55 and TSG101) in E14.5 heterozygous and homozygous the ring, while TEX14 protein localized on the outer rim of the mutant ovaries (Fig. [...] (H) A diagram showing the process of bridge stabilization via TEX14 accumulation on the bridge and the change in its location to the outer layer of the bridge. [...] The thickness of the ovary was obtained by measuring the distance from the top to the bottom of Electron microscopy the tissue. [...] After of each germ cells were measured, and the average value (Rave gc) was used washing the membrane with TBST, the membrane was incubated with an to represent the diameter of the germ cell.