KDM5A and KDM5B histone-demethylases contribute to HU-induced replication stress response

Despite the decrease of the expression of resistance of cells to HU depends on KDM5A- and to a lesser extent these two genes, the cell cycle distribution of U2OS cells was not KDM5B-dependent upregulation of RRM2. [...] Altogether, these data indicate that the expression to sub-lethal doses of HU, and this decrease was reversed by the of KDM5A and KDM5B favours cell survival in the presence of overexpression of wild-type KDM5A, concomitantly with an exogenous replication stress. [...] (A) Relative KDM5A and KDM5B are required for full activation of Chk1 in mRNA expression levels of KDM5A, KDM5B, RRM2, CDC6, CCNE1 and response to RS CHK1 upon transfection of the indicated siRNA in U2OS cells (siCtl The response to RS mostly relies on the activation of the sensor corresponds to a non-targeting pool of siRNA). [...] involved in the management of RS induced by HU, by regulating the levels of RRM2, the regulatory subunit of the ribonucleotide KDM5A localizes at forks and interacts with PCNA and CHK1 reductase RNR, and of CHK1, an effector kinase of RS response. [...] KDM5A regulation the ATR/CHK1 pathway, since the effect of KDM5A and upregulation allows the emergence of cells tolerant to cisplatin, a KDM5B on CHK1 is mainly due to the regulation of its expression.