Department  of  Neurobiology  and  Anatomy

While   highly   effective   at   generating   protein   at   high   purity,   the   buffer   system  we   used   previously   utilized   a   series   of   harsh   chemicals   that   lead   to   rapid   protein   aggregation   during  renaturing  when  attempting  to  upscale  to  larger  batches  of  protein. [...]     Following   confirmation   of   active   protein,   we   next   assessed   rhHsc70’s   therapeutic   viability   in   preserving   neuromuscular   junction   innervation   in   the   SOD1G93A   mouse   model   of   ALS. [...]   Previously,   we   have   shown   that   systemic   administration   of   recombinant   human   Hsp70   beginning   at   postnatal   day   30   delays   peripheral   muscle   denervation   in   this   mouse   model   (Gifondorwa  et  al.,  2012). [...]         Future  Directions   We   are   performing   a   final   series   of   experiments  where   the   protein   is   being   administered   by   intramuscular   injection. [...]         On   behalf   of   all   members   of   our   ALS   Center,   we   continue   to   express   our   gratitude   to   the   Blazeman   Foundation   - ‐   because   of   this   support   we   continue   as   Jon   asked,   we   choose   to   "Believe...