This publication describes the protocols used in the IISD Experimental Lakes Area (IISD-ELA) Analytical Service Laboratory for the measurement of chemical constituents in freshwater samples.
Authors
- Pages
- 274
- Published in
- Canada
- Rights
- IISD, 2024
Table of Contents
- The Chemical Analysis of Fresh Water 1
- The Chemical Analysis of Fresh Water 2
- Abstract 5
- Table of Contents 6
- 1.0 Introduction 7
- 2.0 Labware Cleaning 9
- 3.0 Labware Calibration and Verification 12
- 4.0 Reference Sample Preparation 20
- HOT 20
- 5.0 Laboratory Information Management System Sample Master 30
- Note 35
- Note 35
- Example 37
- 6.0 Sample Preparation and Filtration 41
- HOT 42
- 7.0 Outsourced Analyses 53
- 8.0 Dissolved Oxygen 59
- Never pour water into acid. 60
- Note 61
- Note 64
- Note 64
- Note 65
- Note 65
- Note 65
- Note 65
- 9.0 Specific Conductance 75
- Note 79
- 10.0 Hydrogen Ion Concentration pH 86
- Note 91
- 11.0 Dissolved Inorganic Carbon 97
- Caution 100
- The sparging column can only handle a maximum cumulative volume of 2.0 mL of liquid. 102
- 12.0 Dissolved Organic Carbon Non- Purgative Organic Carbon NPOC 106
- Do not use parafilm to 112
- Note 116
- Note Do not shut down the instrument using the power button on the Shimadzu TOC-VCPH TNM-1 instrument. 117
- Note 119
- Briefly 119
- Immediately 119
- Note 119
- 13.0 Absorbance Scan 127
- Note 131
- 14.0 Nitrite Nitrate and Ammonia Colourimetric method 134
- Never pour water into acid. 134
- Note 140
- Note 141
- Note 144
- Note 146
- Note 146
- Note 147
- 15.0 Total Dissolved Nitrogen UV oxidation and colourimetry method 165
- Never pour water into acid. 165
- Caution This is a dangerous oxidant. 168
- Note 170
- Note 170
- Note 173
- Caution 174
- Note 174
- Note 177
- Note 177
- Note 178
- 16.0 Total Dissolved Phosphorus 192
- Never pour water into acid. 192
- Caution This is a dangerous oxidant. 195
- Caution 196
- Caution 196
- Note 199
- Note 199
- Do not add mixed molybdate to the urea sample. 200
- 17.0 Particulate Phosphorus 211
- HOT 211
- Never pour water into acid. 211
- Caution 214
- Slowly 214
- Caution. 218
- Note HOT 218
- 18.0 Soluble Reactive Silicon Spectrophotometric method 229
- Never pour water into acid. 229
- 19.0 Particulate Iron 245
- Never pour water into acid. 245
- Add 80 mL of Milli-Q water to your volumetric flasks before you add the acid. 248
- Add 80 mL of ultrapure water to your volumetric flask before you add the acid. 248
- 20.0 Chlorophyll- a Gross fluorescence method 256
- Do not use plastic pipette tips as they are wetted with solvent and do not deliver accurate volumes. 260
- Note 261
- Note 262